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Yrophosphate, 1 sodium vanadate, 2.5 EDTA, 2.5 EGTA, 0.05 (w/v) Triton X-100, 0.5 (w/v) SDS, 0.5 (w/v) deoxycholate, 0.5 (w/v) NP-40, 5 mg/ml leupeptin, 5 mg/ml aprotinin, and 1 PMSF. The lysates were centrifuged at 45,000 ?g for 1 h at 4 to yield the whole cell extract. The protein concentration was determined by the BCA reagents according to the instructions of the manufacturer. Samples fr
Ce (NH2-GASGSKARGLWPFAAC) [16]. Formalinfixed and paraffin-embedded tissue histology sections (5 m thick) were deparaffinized, hydrated, and heated in a steamer for 10 min. with 10 mmol/L of sodium citrate (pH 6.0) for antigen retrieval. Peroxide blocking was done with 3 H2O2 in methanol at room temperature for 15 min, followed by 10 bovine serum albumin in TBS-t for 30 min. The slides were inc
Substantia nigra (SN) colocalized with TH in neurons, GFAP in astrocytes and OX-6 and OX-42 in activated microglia [166?68]. Using different carboxy terminal directed AT1 and AT2 antibodies for Western blotting, it was shown that estrogen treatment of ovariectomized rats, which was protective against 6-hydroxydopamine induced neurotoxicity in the SN, decreased AT1 and increased AT2 expression in
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